Microbiology Fundamentals (2nd Edition) Edit edition

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Mixed culture is a laboratory culture that contains two or more different strains of microorganisms. So, in mixed culture, multiple strains or species of bacteria are found in one sample. A contaminated culture is the culture, which should be initially pure, but may have growth of unwanted microbes. So, the contaminated culture is the bacteria culture that contains unwanted bacteria.

To collect urine sample, without being contaminated. The patient should follow the given instructions carefully. Any mishandling during the collection can contaminate the urine sample.

For example, the patient would not have washed her hands, before collecting the sample. Since, our hands contain microflora, touching the urine sample, or the rim of the sample container, without washing hands might contaminate the sample.

Or, the patient might not have collected the midstream portion of the urine. Since, vagina contains different microflora, initial urine, which comes out contains different species of bacteria that might contaminate the urine sample.